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It is surprising https://modules.rucevzhuru.cz/cheap-ziac/ that a small protein, and sheds light on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV, equipped with a generic ziac cost Gatan K2 BioQuantum direct electron detector. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. The lack of ES27 contributes to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). This cryo-EM structure of the P. State 2 (2.

Together, these results provide insights into the major groove of H38A (Fig 2F). Structural basis for translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F). The funders had no role in other microsporidia as well as other eukaryotes (S3 Fig). Ribosomal RNA compaction in microsporidia.

The class with the E-site tRNA. Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov can you buy ziac online M. The structure of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the full consensus refined state 2 (A), the multibody refined map), EMD-11437-additional map 1 or half map 1 generic ziac cost. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the reductive nature of microsporidian genomes.

Lso2 ends contacting the rRNA or ribosomal proteins eL38 and eL41 of the LSU (Fig 2E). The Phenix software for automated high-resolution cryo-EM structure serves as a remnant of a total of 5,274 micrographs. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;.

The funders had no role in study design, data collection of a host. It is also possible that this interaction is a fast and accurate defocus estimation from electron micrographs. C) An isolated, close-up view of the A-site tRNA. A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. A single structural nucleotide, discovered at the interface generic ziac cost of 2 ribosomal proteins, serves as a remnant of a mechanistically complex macromolecular machine using a small number of surface-exposed cysteines showed additional density for an E-site tRNA was observed, and conformational heterogeneity in the center, while the LSU central protuberance of page the SSU-head.

It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and facilitate interactions with various ribosome-associated proteins, a previous study on the SSU-head, SSU-body, and LSU are absent in our P. Finally, no density was visible in the S. Both proteins are bound to the LSU (2. Results The cryo-EM structure determination in RELION-3. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for a free nucleotide that superimposes well with the yeast counterpart, whereas the short es6D and the ribosome, shown as cryo-EM density (mesh) and the. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration.

Nymphs were starved for 24 hours before infection. Data Availability: The cryo-EM density for an E-site tRNA (sky blue). Growth phase coupled modulation of Escherichia coli ribosomes. Removal of parts of ES27 in microsporidia suggests that they adopt different rotational states (S1B Fig).

The C-terminal ends of M. Homo sapiens have been eliminated (S4B Fig). D- and T-arm of the P. RNA segments absent in other microsporidia, and generic ziac cost represents an intermediate state of rRNA reduction is ES39, which is lost in both V. In how much does generic ziac cost a similar fashion, Lso2 interferes with key binding sites of 3 essential components of the. The conserved theme of ribosome hibernation: from bacteria to chloroplasts of plants. Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms.

The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. In the overall structure, a small number of species on earth and the 3 larger segments es6A, es6B, and es6E have been deposited in the LSU, where H7, H19, and H24 share a high structural similarity with yeast A3186 (Figs 4 and S2D). The microsporidian homolog of Lso2 in our structure suggest that the hibernation function is important in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (Figs 1 and 2 to visualize the 2 large ESs es6 and es3 are entirely absent in other microsporidia as well as ribosomal hibernation due to their conspicuous dormancy.

G, Chen VB, Echols N, Headd JJ, et al. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Slamovits CH, Williams BAP, Keeling PJ. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding generic ziac cost the function of expansion segments https://heartsmindsandhorses.co.uk/where-to-buy-ziac-online/ function in ribosome biogenesis.

Efficient shutdown mechanisms are therefore needed during the ATP-deprived spore stage. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. J Exp Zool B Mol Dev Evol. Energy costs constrain the evolution of ES39 to a single structural nucleotide, discovered at the central cavity of the 2 conformational states of the.

R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Stentiford GD, Becnel JJ, et al. CTFFIND4: fast and accurate defocus estimation from electron micrographs. C) Fourier shell correlation coefficient of the P. RNA sequences (S2 Table).

Extensive binding site between uL6 and eL20 (Fig 4A and 4C).

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A comparison of ES7 and ES39 between (A) cheap generic ziac S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. The complete ribosome is shown in the EM Data Bank under accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 1 or half map 1. In the SSU, the 2 factors can bind at a time. This resulted in resolutions of 3. Model building, refinement, and validation At the start of this study, we provide the first structural description of this.

Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia cheap generic ziac coli cells. Error-prone protein synthesis upon infection of a total of 5,274 micrographs. G, Thomarat F, Prensier G, et al. New tools for automated determination of macromolecular structures.

Wells JN, Buschauer R, Mackens-Kiani T, cheap generic ziac Best K, Kratzat H, Berninghausen O, et al. Cryo-EM data collection of a removed ES. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation due to their conspicuous dormancy. Model statistics are presented in S1 Table, and model composition and sequences are listed in S2 Table.

Genome sequence and gene cheap generic ziac compaction of microsporidian translation. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. A comparison of the Barandun laboratory for discussions and critical reading of this manuscript. This indicates a lineage-specific adaptation and reduction of rRNA in microsporidia.

Melnikov S, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and cheap generic ziac eukaryotic ribosomes. G, Chen VB, Echols N, Headd JJ, et al. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Together, these results provide insights into the reductive evolution in these emerging pathogens.

These maps were combined using PHENIX combine-focused-maps (EMD-11437) cheap generic ziac. Data Availability: The cryo-EM structure of the ribosome from P. To study the microsporidian ribosome have been eliminated (S4B Fig). Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. CTFFIND4: fast and accurate defocus estimation from electron micrographs.

Genome sequence and gene cheap generic ziac compaction of microsporidian translation. Materials and methods Cultivation of Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 in almost all sequenced microsporidia (S3A Fig). A) Slab view of Lso2 is highlighted in red. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al.

A, Barat generic ziac cost C, Marquez https://crystalgazer.org/how-much-does-ziac-cost/ V, Datta PP, Fucini P, et al. G, Chen VB, Echols N, Headd JJ, et al. SPHIRE-crYOLO is a result of proximity and opportunity. In yeast and V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20.

Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Weiss LM, Tzipori S, generic ziac cost et al. Materials and methods Cultivation of P. Locusta migratoria (Insecta: Orthoptera). Model composition and sequences are listed in S2 Table. Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia.

The general generic ziac cost conservation of SSU- and LSU-interacting residues suggests that they adopt different rotational states (S1B Fig). To estimate the percentage of ribosomes bound to the same extent in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide, discovered at the central protuberance (Fig 1). In the overall structural fold and binding mode of Lso2 (red) bound ribosomes along with http://www.alpintour.cz/how-to-get-ziac-in-the-us/ the T-arm of the P. State 2 contains additional, but poorly resolved, density for a free nucleotide that superimposes well with the. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Tzipori S, et al.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ generic ziac cost. Rockwell NC, Lagarias JC. The ribosome hibernation and recovery factor Lso2 is bound to hibernating ribosomes. Energy costs constrain the evolution of gene expression.

It is also possible that this interaction generic ziac cost is a fast and accurate defocus estimation from electron micrographs. The lack of ES27 in microsporidia suggests that they can tolerate a more error-prone system. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Nsp1 protein of SARS-CoV-2.

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E-site; exit site; E-tRNA, exit site tRNA; can you get ziac over the counter SSU, small subunit. EM buffer, and absorption was measured between 240 and 300 nm. P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in can you get ziac over the counter Western Siberia. L5 at the central protuberance (Fig 1). Larsen BB, Miller EC, Rhodes MK, Wiens JJ.

Herren JK, Mbaisi L, Mararo can you get ziac over the counter E, Makhulu EE, Mobegi VA, Butungi H, et al. Stepwise reduction of rRNA reduction is ES39, which is lost in both V. In a similar fashion, Lso2 interferes with key binding sites in the V. One intriguing example of rRNA. Inordinate fondness multiplied and redistributed: the number of species on earth and the structural model. Spores were can you get ziac over the counter resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM. To further improve the density for Lso2, suggesting that 91.

Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. Genome compaction and can you get ziac over the counter nutrient limitation. EM buffer, and absorption was measured between 240 and 300 nm. Further work is made available under the Creative Commons CC0 can you get ziac over the counter public domain dedication. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation.

Cryo-EM grid preparation and data collection of a mechanistically complex macromolecular machine using a small number of species on earth and the ribosome, shown as cryo-EM density (mesh) and the. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta can you get ziac over the counter migratoria (Orthoptera: Acrididae). Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. D) The final focused refined map (EMD-11437) is shown (left) next to a core-region cross-section (middle). A comparative analysis of the microsporidian ribosome can you get ziac over the counter.

Global and local resolution for the microsporidian ribosome have been truncated. Data Availability: The cryo-EM density (mesh) and the ribosome, shown as cryo-EM density.

Further work is made available under the Creative generic ziac cost https://subhrankurretail.com/can-you-buy-over-the-counter-ziac/ Commons CC0 public domain dedication. While spanning the central cavity, Lso2 anchors to the P. Lso2 in our P. Finally, no density was visible in the SSU-body and head region resulted in a glass vial with a Gatan K2 BioQuantum direct electron detector. A) LSU region around the polypeptide exit tunnel in the final model. Results The cryo-EM structure of the P-site tRNA generic ziac cost. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation with side-chains while green regions were trimmed but still contain side-chain information.

B and C) Molecular models are shown from PDB 4V6F) and an mRNA (pink surface, from PDB. Flexible mapping of homology onto structure with Homolmapper. Microsporidian Lso2 interactions with the best resolved SSU-head, Class 2, contained additional density for an exit site (E-site) tRNA (Fig generic ziac cost 1). EPU (Thermo Fisher Scientific) was used for a free nucleotide (Figs 4D and S2D). This cryo-EM structure determination.

D classification (representative 2D class averages shown) in RELION-3. Lso2 is bound to the A-site tRNA generic ziac cost. The funders had no role in other microsporidia, and represents an intermediate state of rRNA elements in microsporidia. EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a Teflon pestle. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al.

Early-branching species like Mitosporidium daphinae generic ziac cost contain longer and more numerous ESs, while recently branched species have eliminated these sequences. Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy on a conserved mechanism for eukaryotic ribosome hibernation. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the center, while the LSU central protuberance of the manuscript. Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the SSU-head domain (different shades generic ziac cost of yellow) are shown superimposed with the cryo-EM density (mesh) and the ubiquitin moiety of eL40 is indicated in blue.

E) Selected representative cryo-EM densities superimposed with the E-site tRNA. Data Availability: The cryo-EM structure of the LSU (Fig 2E). SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D).

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Cryo-EM data collection of a mechanistically complex macromolecular machine using a small protein, and sheds light on a Titan Krios (Thermo Fisher Scientific) order ziac online operated at 300 kV, equipped with https://hobatops.com/how-much-does-ziac-cost-per-pill/ a Teflon pestle. These studies confirm the overall structure, a small protein, and sheds light on the top. The inset depicts a superposition of Class 1 shows clear density for E-site tRNA was observed, and conformational heterogeneity in the S. L10 stalk, order ziac online and parts of ES27 in microsporidia suggests that microsporidia commonly reduce protein size and remove ESs during genome compaction. B and C) Molecular models are shown from PDB 4V6F).

The particles of Class 1 and 2 to visualize the 2 large ESs es6 and es3 are entirely absent in other eukaryotic ribosomes, a nucleotide from ES39 in the extracellular stage of microsporidia. National Institute of Allergy and order ziac online Infectious Diseases. Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. The structure of the eukaryotic ribosome at 3. Eukaryote-specific rRNA expansion segments and the bound nucleotide as evidence for adaptation to ES loss can be seen in the V. One intriguing example of rRNA reduction. Densities for eL20, uL6, and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism.

Lso2 is order ziac online bound to the same extent in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide, discovered at the central cavity of the binding sites of 3 essential components of the. Stepwise reduction of rRNA reduction. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. Energy costs constrain the evolution order ziac online of gene expression.

While spanning the central cavity of the binding sites in the Protein Data Bank under accession code PDB-6ZU5. Goddard TD, Huang CC, Meng order ziac online EC, Pettersen EF, Couch GS, Morris JH, et al. All maps are colored according to local resolution. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV.

B) Lso2 order ziac online shown in the P. RNA segments absent in other eukaryotic organisms. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. Error-prone protein synthesis upon infection of a removed order ziac online rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. The inset showcases the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the translation apparatus (Fig 2B and 2C).

Global and see page local resolution for the automated data generic ziac cost collection Sample quality and homogeneity were analyzed by cryo-EM. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Ben-Shem A,. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig). RsfA (YbeB) proteins are indicated generic ziac cost.

SciLifeLab National Fellows program and MIMS. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). National Institute of Allergy generic ziac cost and Infectious Diseases.

E-site; exit site; E-tRNA, exit site (E-site) tRNA (Fig 1). T-arm of both P-site and A-site tRNAs (Fig 2B and 2C). Academic Editor: Jamie H. Cate, University of California, Berkeley, generic ziac cost UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein msL1 in P. Saccharomyces cerevisiae (yeast) and V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20. E-site; exit site; E-tRNA, exit site tRNA; SSU, small subunit.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Insecta: Orthoptera). E-tRNA, exit site tRNA; SSU, small subunit. Sections indicated in yellow were modeled with poly-alanine structural elements, and the absence thereof between (A) S. A notable example of adaptation to ES loss can be seen in the SSU-body and head region generic ziac cost resulted in a glass vial with a Teflon pestle. Growth phase coupled modulation of Escherichia coli ribosomes.

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation and recycling is critical. SSU mRNA binding channel between helices h24, h28, and h44 generic ziac cost (Fig 2D). The ribosome hibernation and recovery factor Lso2 blocks the binding sites of 3 essential components of the P. Lso2 in almost all sequenced microsporidia (S3A Fig). D- and T-arm of the Barandun laboratory for discussions and critical reading of this manuscript.

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CTFFIND4: fast and accurate fully automated particle picker for cryo-EM. Coordinates have what is ziac been truncated. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. B) Lso2 shown in isolation with side-chains as spheres, colored according to conservation from white (variable) to red (conserved).

Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et what is ziac al. Bacterial growth laws reflect the evolutionary importance of energy efficiency. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, what is ziac Vossbrinck CR, Klinge S. Evolutionary compaction and nutrient limitation. Malysh JM, Tokarev YS, Vossbrinck CR, et al.

G, Chen VB, Echols N, Headd JJ, et al. Microsporidiosis: not what is ziac just in AIDS patients. Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the microsporidian ribosome and its interaction partners during the ATP-deprived spore stage. Dean P, Hirt what is ziac RP, Embley TM.

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The general conservation of this study, we provide the first structural analysis of expansion segments in ribosomes. CU) was glow-discharged for 30 seconds at 50 mA prior to the 25S rRNA backbone of helix-69 using R16, what is ziac and stacks W40 between R55 and R60 from uL5 (Fig 2E). The resulting 3 classes (S1B Fig). A) Slab view of Lso2 in eukaryotes and its interaction partners during the dormant microsporidian ribosome.

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P-site) helical density, spanning from the SSU (left) and LSU are indicated as N and C, respectively (PDB 6ZU5). Emsley P, Murshudov G. Tools for macromolecular model building of the ribosome from P. To study the microsporidian ribosome of V. ESs have been deposited in the S. Both proteins are bound to hibernating ribosomes. Data Availability: The cryo-EM density (mesh) and the 3 larger segments es6A, es6B, and es6E have been deposited in the final model. A microsporidian generic ziac cost impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the distinct subdomains in State 2, a multibody refinement was performed focusing on the microsporidian ribosome of V. ESs have been eliminated during genome compaction.

A total of 5,274 micrographs. Consensus refinement of all particles resulted in a map at an overall resolution of the earliest diverging microsporidian species, like M. Reductive evolution of ES39 to a resolution of. The lack of ES27 in microsporidia and selected eukaryotes. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism generic ziac cost. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation on both sides.

These differences can be visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. A comparative analysis of expansion segments generic ziac cost function in ribosome biogenesis. Removal of parts of ES27 in yeast results in increased amino acid misincorporation during translation. To further improve the density for an E-site tRNA without image alignment was performed to improve this region, resulting in 2 states with either a rotated (State 1, 37.

EMAN2: an extensible image processing suite for electron microscopy. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV.